Fig. 3
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Fig. 3 Leukocyte infiltration of epidermis and phagocytosis of debris in clint1 mutants. (A-B′) Wild-type (A,B) and clint1 mutant (A′,B′) zebrafish embryos immunolabeled at 48 hpf for Mpo (green, arrowheads) and L-plastin (red, arrows) (A,A′) or for Mpo (green, arrowheads) and p63 (red) (B,B′). (A,A′) Mpo-expressing cells also express L-plastin and appear yellow. Leukocytes in clint1 mutants infiltrate the caudal fin fold (A′,B′), the lateral epidermis covering the trunk (B′) and dorsal ridge (A′,B′). (B,B′) Confocal yz projections oriented with left side of the embryo facing to the right, dorsal up. Boxed region, CHT. (C,D) Overlay (C) and fluorescence stills (D) from time-lapse movie showing phagocytosis of cellular debris (white arrowhead) by a macrophage (arrow) in lateral epidermis of clint1 mutant at 3 dpf (see Movie 1 in the supplementary material). Yellow arrowhead identifies a neutrophil. Scale bars: 200 μm in A′; 50 μm in D.