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Fig. S7 Keratinocytes and ionocytes of maternal-zygotic epcam mutants mutants express markers of terminal differentiation. To study whether EpCAM is required for terminal differentiation of epidermal cells, maternal/zygotic epcam mutants (MZ-/-; B,D) and wild-type controls (WT; A,C) were stained at 1 dpf for ATPase1b1b and ATPase6v1al transcripts, markers of two ionocytes subtypes, differentiated osmoregulatory skin cells that stem from the same pool of epidermal progenitors like keratinocytes (A,B), or at 3 dpf for keratin protein, a marker for differentiated basal keratinocytes of zebrafish larvae [74] (C,D). Mutants displayed normal signal intensities, normal numbers of ionocytes (A,B), and normal keratin distribution in basal cells (C,D), indicating that EpCAM is dispensable for the differentiation of epidermal cells. In addition, keratin distribution in primary neuromasts appeared normal (indicated by red arrows in C,D).