Fig. S5 Knockdown of tight junction proteins 1–3 fails to restore ruffle formation in epcam mutants. Fluorescent confocal images of phalloidin stained wild type (WT) and maternal-zygotic EpCAM mutant (MZ-/-) embryos. Single and collective knock down of tight junction proteins 1–3 via antisense morpholino oligonucleotides (MO) injections into MZ-/- embryo (panel 2) failed to restore the protrusive activity of the mutant EVL cells back to the WT levels (panel 1, red arrows point to ruffles). If at all, rescue was very minor (orange arrow in panel 3 points to one of the few and rather small ruffles found in 12 investigated tjp1–3 MO-injected embryos).
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