ZFIN Image: Slanchev et al., 2009, Fig. S2

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Figure Caption

Fig. S2 MZepcam mutants display increased susceptibility to cutaneous infections. All panels show lateral views on the tail region of embryos at 2 dpf, after whole mount in-situ hybridization for leukocyte-specific-plastin (lcp1) mRNA, a marker for leukocytes (macrophages and neutrophiles) [59]. (A,B) In wild-type (WT; A) and maternal-zygotic epcam mutants (MZ-/-; B) kept in semi sterile medium, leukocytes are found mainly in the blood vessels. (C,D) Addition of E. coli to the incubation medium stimulates the presence of innate immune cells in the skin (indicated by blue arrows in wild-type fish; C). Skin inflammation is much stronger in the challenged mutant (D) than in the wild-type control (C), suggesting that the mutant is more susceptible to cutaneous infections.

Acknowledgments

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