|
Fig. 2 Assembly of thick filaments in the embryonic zebrafish heart. (A) Thick filaments in embryos at different developmental stages as revealed by immunostaining for myosin heavy chain (MHC) using the F59 antibody. (B) Shown is the two-color immunostaining of 15 S embryos to reveal myosin filaments with the F59 antibody (green) and the F-actin filament network by phalloidin (red). Myosin rodlets and actin filaments do not overlap at this stage. (C) The distribution of lengths of myosin rodlets at 12 S and 24 S, and A-bands at 48 hpf. The myosin rodlets are of variable length until 24 S, when they turn uniform and become shorter at 48 hpf. The x-axis represents the length of myosin rodlets, while the y-axis represents the percentage of myosin rodlets with a particular length. N, total number of myosin rodlets quantified at each stage. Open arrowheads, myosin rodlets of variable length. Arrowheads, myosin rodlets of uniform length after assembling into the thin filament network. Arrows, mature A-bands. Brackets, continuous thin filaments. Insets are same images at higher magnification. Scale bar = 20 μm.
Reprinted from Developmental Biology, 331(2), Huang, W., Zhang, R., and Xu, X., Myofibrillogenesis in the Developing Zebrafish Heart: A Functional Study of tnnt2, 237-249, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.