ZFIN Image: Lamont et al., 2009, Fig. S1

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Figure Caption

Fig. S1 RT-PCR of sema3e^e3i3 and plxnB2^i10e11 morphants.
(A) RT-PCR of sema3e^e3i3 morphants indicating complete mis-splicing of sema3e with 1.9 ng of morpholino. (B) RT-PCR of plxnB2i10e11 morphants indicating complete mis-splicing of plxnB2 with 16 ng of morpholino. EF1α was used as a loading control. (C-D) Schematic representation of the domain structures of both Sema3e (C) and PlxnB2 (D) and resulting protein likely present in sema3e^e3i3 and plxnB2^i10e11 morphants. Both Sema3e and PlxnB2 contain semaphorin and PSI (plexin, semaphorin, integrin) domains. In addition, PlxnB2 contains three IPT (integrin-like, plexin, transcription factors) domains, a transmembrane (TM) domain, and a cytoplasmic signalling domain.

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Reprinted from Developmental Biology, 331(2), Lamont, R.E., Lamont, E.J., and Childs, S.J., Antagonistic interactions among Plexins regulate the timing of intersegmental vessel formation, 199-209, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.