Fig. S2

Fig. S2 Injection of Apc1, but not control MO, causes exacerbation of the apc mutant phenotype. All embryos at 48 hpf. Lateral view. Anterior to the left. In uninjected embryos, only wild-types (n = 38) and (‘mild’) apc (n = 18) phenotypes were observed. Injection of 0.25 mM apc1 MO into progeny of apc^+/- fish causes exacerbation of the apc phenotype (E–G), with 26% of injected embryos (total n = 61) showing no phenotype (E), 56% showing a ‘mild’ phenotype (F) and 18% showing a ‘severe’ phenotype (G). In contrast, injection of 0.25 mM of a 4 mismatch (4mm) control MO into progeny of apc^+/- fish did not affect the apc phenotype as only wild-type (C, n = 50) and ‘mild’ apc phenotypes (D, n = 10) were observed. In 4mm-injected wild-type and apc mutants, the size of the eye was somewhat reduced, indicating a non-specific effect of 4mm MO injection. Scale bar 250 μm.