ZFIN Image: Yabe et al., 2009, Fig. S3

Image

Figure Caption

Fig. S3 aurB^hi1045 homozygotes exhibit an increase in cell apoptosis. (A,B) Exposure to the live cell apoptosis dye acridine orange brightly labels aurB^hi1045 homozygotes (B), while control siblings only exhibit background levels of labeling (A). (C–D) Embryos from incrosses between aurB^hi1045 heterozytes, 25% of which are expected to be aurB^hi1045 homozygotes, were treated with either the caspase inhibitor Boc-D-FMK (D,D′) or control solvent (DMSO; C,C′). Embryos exhibiting the brain necrosis phenotype characteristic of aurB^hi1045 homozygotes are indicated with an asterisk. Boc-D-FMK treatment prevents both the brain necrosis visible under standard microscopy (DIC; D, compare to C) and the accumulation of acridine orange labeled cells (AO; D′ compare to C′).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.