ZFIN Image: Yabe et al., 2009, Fig. 5

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Figure Caption

Fig. 5 Maternal expression of wild-type aurora B kinase rescues the cytokinesis defects in cei mutant embryos.

Embryos from cei/cei homozygous mutant females show a strong syncytial phenotype at the 1,000 cells stage (A) and show defective accumulation of β-catenin in mature furrows at the 8-cell stage (C). Embryos from sibling cei/cei homozygous mutant females which carry transgenic copies of a maternally-expressed wild-type zebrafish aurB gene show significant rescue of the syncytial (B) and β-catenin furrow accumulation phenotype (D). Side views of 1,000-cell stage live embryos (A,B) and animal views of embryos fixed at the 8-cell stage and labeled with an anti-β-catenin antibody (green) and a DNA dye (blue) (C,D). Homozygosity for cei and presence of the transgene were determined by genotyping as in Materials and Methods.

Acknowledgments

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