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Fig. S3 (A) Inhibition of proliferation with HUA resulted in decrease in apoptotic cells in axin1/apc1 embryos. The increase in apoptosis in HUA-treated wild-type embryos, as shown with TUNEL assay, suggests that proliferation has to be tightly regulated. (B) Sustained expression of MyoD+ muscle precursors at 36 hpf, as shown with WISH. Scale bar, 100 μm. (C) Sustained expression of Myogenin+ terminally differentiating cells at 25 hpf, as shown with WISH. Scale bar, 100 μm. (D) Increase in MyoD+ myoblasts at 36hpf, as shown with immunolabelling with anti-MyoD.
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