Fig. 3

Fig. 3 Expression of plce1 at 24 hpf. (A) Lateral view showing the expression of plce1 in the brain, particularly two stripes in the hindbrain and in the developing notochord at 16 hpf. (B) Dorsal view showing that plce1 is expressed extensively in the brain, the eyes and the midbrain–hindbrain boundary (MHB) (arrow). (C) Dorsal view showing that plce1 is expressed strongly in the MHB (arrow) and two rhombomeric segments in the hindbrain (r3 and r5). (D) Lateral view of the trunk showing that plce1 is expressed throughout the somites with a rostral-to-caudal gradient. Inset: transverse section showing plce1 expression in superficial muscle layer and the horizontal myoseptum at 24 hpf. sc: spinal cord. (E) Representative images showing plce1 (red) is expressed in muscle pioneer cells (monoclonal antibody 4D9, green) in somitic segments 4–6 and 21–22 but not in the caudal-most segments 23–24 at 24 hpf. ss: somitic segments. (F) Lateral view of the tail region showing plce1 expression is high at the tip of the extending notochord and the developing cloaca (arrow). Inset: sagittal section of the cloaca region. (G–I) Transverse sections of 24 hpf, 32 hpf, and 36 hpf embryos showing the plce1 expression in developing pronephric glomerular primordium (arrows).