ZFIN Image: Tucker et al., 2008, Fig. S1

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Figure Caption

Fig. S1 P-Smad5 Is Expressed in Ectodermal and Mesendodermal Cells on the Ventral Side of the Embryo during Gastrulation
(A, D, G, J) P-Smad5 expression. (B, E, H, K) Nuclear staining using TO-PRO-3 (Molecular Probes). (C, F, I, L) Overlay of above panels. P-Smad5 is expressed in nearly all cells on the ventral side of the embryo at all positions along the animal-vegetal axis at 6 hpf (A, B, C) and 7 hpf (G, H, I), but is not expressed in any cells on the dorsal side at the same time points (D, E, F, J, K, L). Arrowheads denote involuted mesendodermal cells expressing P-Smad5. All panels are 1 μM thick confocal slices, lateral views with animal pole to the top.

Acknowledgments

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Reprinted from Developmental Cell, 14(1), Tucker, J.A., Mintzer, K.A., and Mullins, M.C., The BMP signaling gradient patterns dorsoventral tissues in a temporally progressive manner along the anteroposterior axis, 108-119, Copyright (2008) with permission from Elsevier. Full text @ Dev. Cell