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Fig. 6 Progressive BMP Inhibition Series Reveals Progressive DV Mesodermal Patterning along the AP Axis, and Rescue of Posterior, but Not Anterior, in MZ-laf Embryos
(A–L) WT1 expression in the pronephros in heat-shocked nontransgenic WT siblings (A), and following HS at 5 hpf ([D], n = 16/19), 5.5 hpf ([G], n = 7/12), or 6 hpf ([J], n = 10/12). pax2.1 expression in pronephros (arrowheads), MHB (arrow), and otic placode (asterisk) in WT (B), and following HS at 5 hpf ([E], n = 16/16), 5.5 hpf ([H], n = 14/24), or 6 hpf ([K], n = 10/13). krox20 expression was also examined in ([H], bracket). Expression of gata1 in blood progenitors (arrowheads) and krox20 in hindbrain (brackets) in WT (C), and following HS at 5 hpf ([F], n = 5/5), 5.5 hpf ([I], n = 6/6), or 6 hpf ([L], n = 5/7).
(M and N) krox20 expression in R3 and R5 (arrowheads), and myoD in somitic mesoderm in MZ-laf mutants overexpressing bmp2b in the absence of HS (M) or following a 30 min heat-shock induction at 6.75 hpf (N) of Tg(hsp70:alk8) causes partial expansion of R5 (asterisk) but complete expansion of R3 (arrowhead), and reinitiation of P-Smad5 by 7.25 hpf (O).
(A)–(N) are lateral views, dorsal to right, at the five-somite stage. (O) is animal pole view, dorsal to right.
Reprinted from Developmental Cell, 14(1), Tucker, J.A., Mintzer, K.A., and Mullins, M.C., The BMP signaling gradient patterns dorsoventral tissues in a temporally progressive manner along the anteroposterior axis, 108-119, Copyright (2008) with permission from Elsevier. Full text @ Dev. Cell