ZFIN Image: Tucker et al., 2008, Fig. 3

Image

Figure Caption

Fig. 3 Tg(hsp70:chd) Rapidly Inhibits BMP Signaling, Generating a Range of Dorsalized Phenotypes Dependent on Induction Time

(A) P-Smad5 western blot at blastula (3.0–5.25 hpf), onset of gastrulation (5.5 hpf) through midgastrulation (5.75–7.75 hpf) stages.

(B) P-Smad5 during (20 and 40 min time points) and immediately after a 60 min HS at 4, 5, or 6 hpf. Actin is a loading control.

(C–F) Nontransgenic WT (C) and transgenic siblings heat-shocked at 4 hpf (D) or 6 hpf (E) at the three-somite stage and at 1 day postfertilization (dpf) (F).

(G and H) Dorsalization at 1 dpf of embryos heat-shocked at 7 hpf (G) and 10 hpf ([H], arrows indicate loss of ventral tail tissue).

(I) Distribution of dorsalized phenotypes following HS at different time points.

Acknowledgments

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Reprinted from Developmental Cell, 14(1), Tucker, J.A., Mintzer, K.A., and Mullins, M.C., The BMP signaling gradient patterns dorsoventral tissues in a temporally progressive manner along the anteroposterior axis, 108-119, Copyright (2008) with permission from Elsevier. Full text @ Dev. Cell