ZFIN Image: Bae et al., 2009, Fig. 10

Image

Figure Caption

Fig. 10 Presynaptic terminals of granule neurons (parallel fibers) and GABAergic interneurons. (A) Schematic presentation. (B) Immunoblotting of brain lysates with the anti-Vglut1 antibody. (C–E) Co-staining with anti-Pvalb7 (green) and anti-Vglut1 (magenta) antibodies. (C–E) Sagittal sections with anterior to the left; low (C), medium (D), and high (E) magnification views. (D, E) Corpus cerebelli (CCe). Vglut1⁺ dots (presynaptic boutons of parallel fibers) abutted the dendrites but not the soma (asterisk) of Purkinje cells in the ML. (F, G) Co-staining with anti-Pvalb7 (green) and anti-Gad1 (magenta) antibodies. Gad1⁺ dots (presynaptic boutons of GABAergic interneurons) abut the dendrites and somata (asterisks) of Purkinje cells. The abbreviations are as described in Fig. 1. Scale bars: 200 μm (C), 100 μm (F) and 20 μm (D, E, G).

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Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Biology, 330(2), Bae, Y.K., Kani, S., Shimizu, T., Tanabe, K., Nojima, H., Kimura, Y., Higashijima, S.I., and Hibi, M., Anatomy of zebrafish cerebellum and screen for mutations affecting its development, 406-426, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.