ZFIN Image: Bae et al., 2009, Fig. 2

Image

Figure Caption

Fig. 2 GABAergic neurons. (A–F) Expression of gad1 (A), gad2 (B), carbonic anhydrase 8 (ca8, C), lhx1a (lim1, D), aldolase c-like (aldocl, E), and parvalbumin7 (pvalb7, F) in the adult cerebellum. In situ hybridization of sagittal sections. Anterior is to the left. (G, H) Comparison of pvalb7 (green) and gad1 (magenta) expression. (H) High-magnification view of the box in G. Note that all the pvalb7⁺ cells are gad1⁺ (white), but there are gad1⁺ pvalb7^- cells (magenta) in the GCL and ML. (I, J) Fluorescence immunohistochemistry of adult cerebellum with an anti-parvalbumin7 antibody (Pvalb7). Low (I) and high (J) magnification views. The position of the PCL is indicated by an arrowhead (I). Axons of Purkinje cells are indicated by arrows (J). Pvalb7⁺ axons reach and surround Pvalb7^- cells (indicated by arrows), which correspond to eurydendroid cells (J). (K–N) Anti-carbonic anhydrase 8 (Ca8, K), zebrin II (L) and anti-parvalbumin7 (Pvalb7, M) antibody staining. Zebrin II (green) and Pvalb7 (magenta) staining completely overlap (N). The abbreviations are described in Fig. 1. Scale bars: 500 μm (F, G, I) and 50 μm (H, J, N).

Figure Data

Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Biology, 330(2), Bae, Y.K., Kani, S., Shimizu, T., Tanabe, K., Nojima, H., Kimura, Y., Higashijima, S.I., and Hibi, M., Anatomy of zebrafish cerebellum and screen for mutations affecting its development, 406-426, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.