ZFIN Image: North et al., 2009, Fig. 4

Image

Figure Caption

Fig. 4 NO Signaling Affects Zebrafish HSC Formation Independent of Heartbeat

(A–I) WT and sih^-/- mutants were exposed to DMSO and SNAP (10 μM) from 10 somites–36 hpf.

(A–D) In situ hybridization for runx1/cmyb. SNAP rescues HSC formation in sih^-/- mutants.

(B) Remaining runx1/cmyb+ cells are highlighted by arrowheads.

(E) qPCR for runx1. ^* statistically significant versus the WT, mean ± SD, ANOVA, p < 0.001, n = 5.

(F–I) Effect of heartbeat and SNAP on ephrinB2 expression, highlighted by arrowheads.

(J–U) Effect of L-NAME on HSC formation in embryos concurrently treated with blood flow-modifying agents. L-NAME inhibits the effects of doxazosin ([M], 7 inc/36 observed), metoprolol ([O], 3 inc/31) and nifedipine ([Q], 4 inc/28), but not of digoxin ([S], 16 inc/ 29) and todralazine ([U], 20 inc/33).

Figure Data

Expression / Labeling details

Acknowledgments

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Reprinted from Cell, 137(4), North, T.E., Goessling, W., Peeters, M., Li, P., Ceol, C., Lord, A.M., Weber, G.J., Harris, J., Cutting, C.C., Huang, P., Dzierzak, E., and Zon, L.I., Hematopoietic stem cell development is dependent on blood flow, 736-748, Copyright (2009) with permission from Elsevier. Full text @ Cell