ZFIN Image: Yeo et al., 2001, Fig. 7

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Figure Caption

Fig. 7 Localized ectopic expression of Slit2 impairs the mesodermal movement. The embryos expressing slit2 and gfp mRNA in a mosaic pattern were fixed at 10 h and stained for hlx1 and dlx3 expression by in situ hybridization and for GFP by immunohistochemistry. Dorsal views, anterior to the top. (A) Control embryo. (B) The embryos (n 5 3) in which the GFP-positive cells were broadly distributed in the anterior region of the neural plate. The hlx1-positive cells of the prechordal mesoderm failed to reach the anterior margin of neural plate. (C) The embryos (n 5 17) in which the GFP-positive cells were incorporated on one side of the neural plate and the adjacent ectoderm. The hlx1-positive cells were bent toward the side which incorporated the GFP-positive cells. Bar, 250 μm.

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Reprinted from Developmental Biology, 230(1), Yeo, S.Y., Little, M.H., Yamada, T., Miyashita, T., Halloran, M.C., Kuwada, J.Y., Huh, T.L., and Okamoto, H., Overexpression of a slit homologue impairs convergent extension of the mesoderm and causes cyclopia in embryonic zebrafish, 1-17, Copyright (2001) with permission from Elsevier. Full text @ Dev. Biol.