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Fig. 5 Overexpression of slit2 mRNA induces cyclopia in the zebrafish embryo. (A–F) Various degrees of cyclopia were observed in the slit2-overexpressing embryos at 16 h (B, C) and 48 h (E, F). A and D, normal embryos. Embryos are shown in dorsal views (A–C) and in frontal views (D–F). (G–J) Defect in the ventral forebrain of 16-h zebrafish embryo overexpressing slit2 mRNA. (G, H) Expression of shh (G) and opl (H) mRNA in normal embryos. (I, J) Expression of shh (I) and opl (J) mRNA in embryos overexpressing slit2 mRNA. In the slit2-overexpressing embryo, the anterior edge of the shh-positive domain in the ventral forebrain (vf) is caudally shifted as indicated by the arrow in (I). In contrast, opl mRNA is expressed indistinguishably in the dorsal forebrain of the normal embryo (H) and the embryo overexpressing slit2 mRNA (J). e and arrows, eyes; pe and arrowheads, the retinal pigment epithelium; t, telencephalon; fp, floor plate cells. Bars, 200 μm (A–F, G–J).
Reprinted from Developmental Biology, 230(1), Yeo, S.Y., Little, M.H., Yamada, T., Miyashita, T., Halloran, M.C., Kuwada, J.Y., Huh, T.L., and Okamoto, H., Overexpression of a slit homologue impairs convergent extension of the mesoderm and causes cyclopia in embryonic zebrafish, 1-17, Copyright (2001) with permission from Elsevier. Full text @ Dev. Biol.