Fig. 5 Posterior displacement of the polster in slb is accompanied by a shortening of the shh expression domain at bud stage. (A, C) Double labeling staining the polster with an anti-Fkd2 antibody (brown) and the anterior edge of the neural plate by its expression of dlx3 (blue) in a wild-type (A) and slb (C) embryo at bud stage. (B, D) In situ staining which labels the midline (shh) and anterior edge of the neural plate (dlx3) in a wild-type (B) and slb (D) embryo at bud stage. Arrowheads in D demarcate the gap between the anterior edge of the neural plate and the anterior end of the shh expression domain in slb. Dorsal views, anterior to the left.
Reprinted from Developmental Biology, 184(1), Heisenberg, C.P. and Nüsslein-Volhard, C., The function of silberblick in the positioning of the eye anlage in the zebrafish embryo, 85-94, Copyright (1997) with permission from Elsevier. Full text @ Dev. Biol.