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Fig. 2 Expression of Lim3 is limited to the pituitary, epiphysis, hindbrain, and ventral spinal cord in 28-h zebrafish embryos. The expression of Lim3 protein in zebrafish was determined by whole-mount immunohistochemistry using anti-Lim3 antibody. At 28 h postfertilization, Lim3 protein expression is restricted to nuclei of the pituitary anlage (p), the epiphysis (e), the hindbrain, and the spinal cord. OT, otic vesicle. The approximate posterior borders of the first four somites are indicated with hash marks. The staining in the eye lens is artifactual, due to nonspecific trapping of the antibody. Scale bar is 100 μm.
Reprinted from Developmental Biology, 192, Glasgow, E., Karavanov, A.A., and Dawid, I.B., Neuronal and neuroendocrine expression of lim3, a LIM class homeobox gene, is altered in mutant zebrafish with axial signaling defects, 405-419, Copyright (1997) with permission from Elsevier. Full text @ Dev. Biol.