ZFIN Image: Thompson et al., 1998, Fig. 5

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Figure Caption

Fig. 5 Deletion of the c-myb gene in b316 mutants does not result in a loss of gata1 expression in zebrafish embryonic erythroid cells. Whole-mount in situ hybridization of wild-type (A) and b316 (B) embryos at approximately 18 h postfertilization with gata1. Note that the b316 embryo development is arrested at about 5-somites, but that it appears to have a normal level of gata1 expression. PCR of DNA from wild-type or b316 embryos with two different c-myb primer pairs, and with primers specific for gata2, lmo2, and gata1 (C). c-myb is not amplified with either primer pair from DNA made from b316 embryos, while it is amplified from DNA made from wild-type embryos. gata2, lmo2, and gata1 all amplify in both b316 and wild-type embryos.

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Acknowledgments

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Reprinted from Developmental Biology, 197, Thompson, M.A., Ransom, D.G., Pratt, S.J., MacLennan, H., Kieran, M.W., Detrich,III, H.W., Vail, B., Huber, T.L., Paw, B., Brownlie, A.J., Oates, A.C., Fritz, A., Gates, MA., Amores, A., Bahary, N., Talbot, W.S., Her, H., Beier, D.R., Postlethwait, J.H., and Zon, L.I., The cloche and spadetail genes differentially affect hematopoiesis and vasculogenesis, 248-269, Copyright (1998) with permission from Elsevier. Full text @ Dev. Biol.