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Fig. 7 ehh and twhh are present in single mutants but absent from the double mutant. Dorsal views (anterior to the top) of wholemount RNA in situ hybridizations at the 3-somite stage (11 hr). (A) flh mutant embryo hybridized with an antisense probe against twhh. (B) cyc mutant embryo hybridized with an antisense probe against ehh. cyc-;flh- double mutants’ lack both twhh and ehh at this stage (Table 1). Scale bar, 200 μm.
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Reprinted from Developmental Biology, 187(2), Beattie, C.E., Hatta, K., Halpern, M.E., Liu, H., Eisen, J.S., and Kimmel, C.B., Temporal separation in the specification of primary and secondary motoneurons in zebrafish, 171-182, Copyright (1997) with permission from Elsevier. Full text @ Dev. Biol.