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Fig. S4 olig1 morpholino reduces olig1 expression and blocks translation of an Olig1-GFP fusion protein. A: Injection of 750 μM olig1 morpholino resulted in reduced mRNA expression of olig1 at 48 hpf compared to wildtype sibling controls. B: Translation of an injected reporter mRNA, containing the olig1-MO targeting sequence fused in frame to eGFP, was completely abolished by co-injection of olig1 morpholino at 250 μM or higher, while control-injected embryos without morpholino showed strong GFP activity. Several independent examples for each injection are shown for comparison.
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