Fig. 1 Early ectopic expression of transgenic flk1:gfp is induced by etsrp over-expression.
(A) Injection of 75 pg of etsrp mRNA at one-cell stage results in the ectopic induction of flk1:gfp before the end of gastrulation. (B) No red fluorescence is observed in etsrp mRNA injected flk1:gfp transgenic embryos. (C) Injection of 500 pg of control H2B-RFP1 mRNA does not result in the early induction of flk1:gfp similar to uninjected embryos (not shown). (D) Uniform H2B-RFP1 expression in a H2B-RFP1 mRNA injected embryo. Panels A and C are composite images of light transmitted images merged with the green fluorescent channel, while panels B and D are composites of light transmitted images merged with the red fluorescent channel.