ZFIN Image: Jing et al., 2009, Fig. S6

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Figure Caption

Fig. S6 Formation of neural synapses can occur independent of AChR prepatterning.
(A-D) Images of untreated Tg(hsp70l:UnpSV1-myc;unplugged) embryos (A, B), and Tg(hsp70l:UnpFL-myc; unplugged) embryos at the 20-somite stage (A, C) or at 27 hpf (B, D). AChR prepattern and neural synapses (red, α-BTX) are absent, and axons display unplugged-like phenotypes (green, znp-1). (E-G′) High-magnification views of neural synapses in wildtype embryos (E) and ‘rescued’ transgenic embryos. Embryos heat-shock treated between the 26-somite stage and 27 hpf, displayed unplugged-like axons with neural AChRs apposed the length of the axon (G, G′). (H) Cell extracts from individual transgenic embryos, with and without heat shock treatment (38°C for 35 min), were subjected to anti-myc immunoblotting. Scale bars: in C and D: 50 μm; in E: 20 μm.

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