IMAGE

Fig. S5

ID
ZDB-IMAGE-090320-78
Source
Figures for Jing et al., 2009
Image
Figure Caption

Fig. S5 Unplugged interacts with zebrafish Dishevelled.
(A) The intracellular domain of unplugged /MuSK (Unpic) was cloned into a bait plasmid pGBKT7. The PDZ and DEP domains of zebrafish dishevelled 2 and dishevelled 3 were cloned into a prey plasmid pGADT7. Two-hybrid interactions were preformed using SD/-Ade/-His/-Leu/-Trp media to screen for reporter genes: ADE2 and HIS3 (+++: fast growth. ++: intermediate growth. -: no growth). (B, C): The morphology and differentiation of adaxial cells expressing Myc-Xdsh-DEP+ appears normal. 28 hpf embryo injected with symhc1:myc-Xdsh-DEP+ and stained with mAb anti-Myc (red) and F59 (green, adaxial cells).

Acknowledgments
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