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Fig. 6 Neuromuscular Synapses Form in the Absence of AChR Prepattern
Twenty-somite stage (A, B, G, and H) or 27 hpf (C–F, I, and J) embryos after heat-shock treatment.
(A and B) Tg(hsp70l:UnpSV1-myc;unplugged) embryos received heat shock from the 10 to 20 somite stage, which rescued AChR prepattern.
(C and D) Similar heat-shock treatment (10 somite to 27 hpf) also restored motor axon pathfinding, but not neuromuscular synapses.
(E and F) The same heat-shock treatment rescued motor axons and neuromuscular synapses in Tg(hsp70l:UnpFL-myc; unplugged) embryos.
(G and H) In contrast, heat shock between the 10 and 20 somite stage failed to rescue AChR prepattern in Tg(hsp70l:UnpFL-myc;unplugged) embryos.
(I and J) Heat-shock treatment of same embryos between the 26 somite stage and 27 hpf, i.e., after the time period of prepatterning, was sufficient to rescue neuromuscular synapses.
Scale bars, 50 μm.