ZFIN Image: Bontems et al., 2009, Fig. S1

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Figure Caption

Fig. S1 Egg Polarity and Germ Plasm Formation in Early Bucky Ball Oocytes
(A-H) Fluorescent whole-mount in situ hybridization of stage III (A, B) and early Ib oocytes (C-H). cyclinB (A, B), dazl (C, D), vasa (E, F) (red) and foxH1 (G, H) (green) in wild-type (A, C, E, G) and buc^p106re mutant stage I oocytes (B, D, F, H).
(I, J) Living staining of mitochondria and endoplasmic reticulum with DiOC₆ (green) in wt (I) and buc^p106re mutant oocytes (J). Note the absence of signal at the vegetal pole in mutants. Animal pole to the top. Scale bar: 50 μm (A, B), 25μm (C-J).
(K) Quantification of oocytes with localized dazl mRNA. Note dazl mRNA localization in wild type (WT) (94.7±5.5%; n=385; grey bar) but not in buc^p106re mutants (MUT) (4.8±2.6%; n=402; white bar). Error bars indicate the standard deviation of the average (at least three independent experiments).
(L) Quantitative real-time PCR measuring the relative concentration of vasa (white) and dazl (grey) mRNA in whole ovaries from wt and buc^p106re mut females.

Acknowledgments

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