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Fig. 2 Cell sorting in EphA4MO mosaics begins during the neural keel stage. Representative 30 μm-deep projections of adjacent confocal sections through the hindbrain of a live mosaic embryo are shown at the indicated time points. Left panel: distribution of mRFP+ WT donor cells. Middle panel: expression of GFP in r3, r5 of the EphA4 MO pGFP5.3 transgenic host. Right panel is a merge of two channels shown at left. (A) At 11 hpf (neural plate), cells are uniformly distributed on one side of the hindbrain. (B) At 12.5 hpf (neural keel), presumptive daughters of neural progenitor divisions (white arrowheads) have begun to cross the midline (blue dotted line) but none is observed crossing in the r3 or r5 interior. (C, D) By 14.5 hpf (neural rod) and 18 hpf (neural tube), many donor cells have successfully crossed the midline (white arrowheads) except in r3 and r5, where WT mRFP+, GFP- cells form unilateral cell clusters (yellow triangles). OP (otic placode); pMHB (presumptive midbrain–hindbrain boundary). Scale bar: 50 μm.
Reprinted from Developmental Biology, 327(2), Kemp, H.A., Cooke, J.E., and Moens, C.B., EphA4 and EfnB2a maintain rhombomere coherence by independently regulating intercalation of progenitor cells in the zebrafish neural keel, 313-326, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.