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Fig. 1 HGn39D transgenic zebrafish express GFP in the lateral line afferent neurons.
(A–C) GFP expression pattern in a 4 dpf HGn39D transgenic fish. Maximal projections of lateral- (A) and top-views (B) are depicted. White and yellow arrowheads point to the neuronal projections to the hindbrain and to the neuromast respectively. Dashed frames indicate the lateral line ganglia in A and B. (C) Maximal projections of the posterior lateral line ganglion; approximately 25 somata can be counted. (D) Maximal projection of an HGn39D innervated neuromast immunolabeled with anti-HCS1 antibody (red). (E–P) Anti- tyrosine hydroxylase (TH) and Gem25.2/Cav1.3a (Gem) immunostainings were performed on 7 dpf HGn39D fish. (E,H,K,N) HGn39D GFP expression; (F,I) anti-TH antibody labels the efferent neurons; (L,O) Anti-Gem antibody labels the afferent neurons. Maximal projections of the posterior lateral line nerve (E–G, K–M) and of the terminal neuromast of the posterior lateral line (H–J, N–P) are depicted. (G,J,M,P). Overlays of HGn39D GFP expression and specific immunostainings. (Q–S) DiASP is trans-synaptically transported into the HGn39D afferent neurons. (Q) HGn39D GFP expression; (R) DiASP incorporation; (S) Overlay of HGn39D GFP expression and DiASP incorporation. Scale bars: 100 μm (A, B) and 10 μm (C–S).