Fig. 9

Fig. 9 Immunohistochemical analysis of wild type, ift57, ift88 and ift172 mutant zebrafish at 96 hpf. (A–D) 1D1 (green), a marker for rhodopsin, localized to the outer segment region in wild type and ift57 mutants. Strong mislocalization to the inner segment was also observed in ift57, ift88, and ift172 mutants. (E–H) Zpr1 (green), a label for red/green double cones gave an elongated, columnar morphology in wild type animals. Cone morphology was disheveled in the three mutants. (I–J) Blue opsin (BOPS) was localized to the outer segments of wild type photoreceptors. Staining was observed in short outer segments of ift57 photoreceptors but strong mislocalization was seen in the ift88 and ift172 mutants. (M–P) IFT52 (red) co-localized with acetylated tubulin (green) in the connecting cilia of wild type and ift57 mutants (arrows). No such staining was observed in ift88 and ift172 mutants. (Q–T) IFT88 (red) also showed strong apical localization in wild type and ift57 mutants. The staining overlapped with acetylated tubulin (green), in wild type and ift57 animals (arrows). No staining was observed in ift88 and ift172 mutants. (U–X) Centrin (red) localized to the apical surface of the inner segment. Acetylated tubulin (green) denotes microtubules. Centrin was seen in wild type and all IFT mutants. The number of centrin-positive foci was reduced in ift88 and ift172 mutants. Centrin was occasionally observed to be mislocalized in ift172 mutants (Fig. 9X; white arrowheads). In all images, the tissues were counterstained with DAPI (blue). Scale bar = 20 μm.