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Fig. S9 Analysis of otpa overexpression in hs:otpa transgenic fish. (A,B) Whole-mount in situ hybridization for otpa in hs:otpa transgenic embryos at 32 hpf; heatshocks performed at 9 hpf and 28 hpf for 1 hour at 39°C results in ubiquitous otpa expression (A), whereas non-heatshocked embryos display endogenous otpa expression only (B). The endogenous otpa signal in heatshocked embryos is still stronger than otpa signal from the transgene (arrowheads in A). (A,B) Lateral views; anterior is towards the left. Scale bar: 100 μm for A,B.
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