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Fig. 6 Combined overexpression of Otpa and Sim1a leads to supernumerary DA and CRH cells in the ventral diencephalon. (A-H) Whole-mount in situ hybridization. Expression of th at 24 hpf (A,B), 32 hpf (C,D) and 48 hpf (E,F), and crh at 32 hpf (G,H) in wild-type controls (A,C,E,G) compared with transgenic embryos expressing otpa from a hsp70 promoter, and injected with sim1a mRNA (Otp/Sim1OE; B,D,F,H). Only the number of group 2 cells is strongly increased in Otp/Sim1OE embryos, whereas groups 4/6 are not affected (A-F). The number of CRH cells is increased in Otp/Sim1OE embryos (G,H). Lateral views, anterior towards the left. Scale bar: 50 μm for A,B; 62.5 μm for C,D,G,H; 80 μm for E,F.