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Fig. S2 Expression of putative T-box gene targets overlaps with ntl and spt expression during gastrulation. Shown are wild-type in situ hybridization patterns during gastrula stages (6-9 hpf) for genes downregulated at least 2.5-fold in embryos depleted of spt and ntl function. Expression patterns obtained from the ZFIN database (Thisse et al., 2001) are indicated. (A) ntl and spt expression patterns at mid-gastrulation. (B) Expression of 18 potential targets, arranged (from top left to bottom right) to indicate the magnitude to which they were affected (fold decrease indicated at the bottom right of each panel). Two additional targets are expressed in patterns that overlap with spt and/or ntl, but are not shown: during gastrulation, knypek (3.8x) is expressed in the axial mesoderm (Machingo et al., 2006) and six4.2 (2.8x) is expressed in the non-axial mesoderm (Kobayashi et al., 2000). An additional nine potential targets that were down-regulated at 2.5-fold or greater are not shown for the following reasons: the gene they identify is not known four genes: AI942866 (8x); AI584322 (6x); BM036916 (4.5x); and BI886648 (4x), their gastrula expression patterns have not been well characterized or described two genes: cxcl12a (2.8x) and itm2c (2.5x), their gastrula expression levels have been reported as low or not detectable two genes: ywhae1 (3.6x) and efemp2 (3x), or they have been described to be ubiquitously expressed during gastrula stages one gene: tuba812 (2.8x).