ZFIN Image: Garnett et al., 2009, Fig. 6

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Figure Caption

Fig. 6 The tbx6 regulatory element drives mesodermal gene expression and requires Spt and Ntl for activity. The 1.7 kb region upstream of tbx6 is shown with gray rectangles representing predicted Spt- and/or Ntl-binding site motifs (A). This region drives reporter expression at the margin at mid-gastrulation (B), but not if embryos are co-injected with spt and ntl MOs (C) (mCherry, red; GFP, green). (D-G) Endogenous tbx6 expression is decreased in spt- (F) or ntl-depleted (E) embryos when compared with expression in wild-type embryos (D). tbx6 expression is undetectable when both ntl and spt are depleted (G).

Acknowledgments

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