ZFIN Image: Ochocinska et al., 2009, Fig. S1

Image

Figure Caption

Fig. S1 The construct encoding pHsp70/4:neuroD_EGFP is transcribed in vitro. Panel A is a cartoon illustrating subcloning of the Hsp70/4:neuroD^EGFP construct. The Hsp70/4:neuroD_EGFP construct was assembled by subcloning the 1 kb coding region of the zebrafish neuroD gene into the Hsp70/4:EGFP vector. Panels B1-3 illustrate the localization of EGFP in the cytoplasm of HEK293 cells transfected with the empty Hsp70/4:EGFP construct. Panels C1-3 illustrate localization of EGFP in the nucleus of HEK293 cells transfected with the Hsp70/4:neuroD_EGFP construct. Left-hand panels illustrate GFP fluorescence; Middle panels illustrate nuclear staining with bisbenzimide; right-hand panels are digital overlays.

Acknowledgments

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Reprinted from Mechanisms of Development, 126(3-4), Ochocinska, M.J., and Hitchcock, P.F., NeuroD regulates proliferation of photoreceptor progenitors in the retina of the zebrafish, 128-141, Copyright (2009) with permission from Elsevier. Full text @ Mech. Dev.