ZFIN Image: Ochocinska et al., 2009, Fig. 2

Image

Figure Caption

Fig. 2 Induced neuroD promotes exit from the cell cycle exit in vivo, but does not cause cell death. Panel A illustrates a retina from a wt embryo treated with heat shock at 24hpf and labeled with BrdU and sacrificed at 48hpf. Note the extensive BrdU labeling throughout. Panel B is a retina from a transgenic embryo treated with heat shock at 24hpf and labeled with BrdU and sacrificed at 48hpf. Panel C is the retina illustrated in B, showing the BrdU labeling. Panel D is the digital overlay of panels B and C. Panel E illustrates histograms comparing the number of phosphohistone H3 immunopositive cells in the retinas of wt and transgenic embryos. Panel F illustrates histograms comparing the number of TUNEL-positive cells in the retinas of wt and transgenic embryos. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer; CMZ, circumferential marginal zone. Scale bar equals 50 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Mechanisms of Development, 126(3-4), Ochocinska, M.J., and Hitchcock, P.F., NeuroD regulates proliferation of photoreceptor progenitors in the retina of the zebrafish, 128-141, Copyright (2009) with permission from Elsevier. Full text @ Mech. Dev.