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Fig. S2 irx4a has no role in neural crest development. The expression pattern of the neural crest marker foxd3 was analyzed by in situ hybridization (A, B) and GFP expression in the transgenic line foxd3-GFP (C, D) was analyzed in control (A, C) and morphant embryos (B, D) at 24 hpf and 48 hpf, respectively. We detected no differences in the distribution or intensity of both markers. g, glia. All panels show lateral views; dorsal is up, anterior is left. Scale bar in D: 30 μm for C, D.
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Reprinted from Molecular and cellular neurosciences, 40(3), Feijoo, C.G., Saldias, M.P., De la Paz, J.F., Gómez-Skarmeta, J.L., and Allende, M.L., Formation of posterior cranial placode derivatives requires the Iroquois transcription factor irx4a, 328-337, Copyright (2009) with permission from Elsevier. Full text @ Mol. Cell Neurosci.