Fig. S2

Fig. S2 Colocalisation of β-catenin and desmocollin is unaltered in plakoglobin morphant embryos. (A–F) Laser-scanning immunofluorescence microscopy images of transverse sections of the eye region of 72 hpf embryos. Fluorescence images with anti-β-catenin antibody (red), anti-desmocollin 2 and 3 antibody (green) and merged dual images (E and F) are shown. Images are 100x magnification. (A, C, E) In control morpholino injected embryos and (D, E, F) plakoglobin morphant embryos there was little colocalisation of β-catenin and desmocollin 2 and 3. (G) Quantification of co-localisation of E-cadherin and β-catenin in control and morphant embryos. There was a similar level of co-localisation of β-catenin and desmocollin 2 and 3 in control and morphant embryos. Antibody controls for desmocollin 2 and 3 antibody. (H) β-catenin staining in the absence of desmocollin primary antibody. (I) Desmocollin staining in the absence of the desmocollin primary antibody. (J) Merged image of both β-catenin and desmocollin staining. (K) β-catenin expression in the absence of desmocollin secondary antibody control. (L) Desmocollin 2 and 3 staining in the absence of its secondary antibody. (M) Merged image of both β-catenin and desmocollin 2 and 3 staining in the absence of desmocollin secondary antibody. Little staining was detected in either the absence of desmocollin primary or secondary antibody indicating that the primary antibody and the secondary antibody are specific.