Fig. 4

Fig. 4 Expression of cardiac marker genes in plakoglobin morphant and control morpholino injected embryos. (A and B) lateral views of 24 hpf and (C–J) ventral views of 48 hpf wholemount embryos. (A) Nkx2.5 at 24 hpf was expressed to the left of the midline in control injected embryos. (B) Nkx2.5 expression is reduced in morphant embryos but in the correct location. (C) Cmlc2 is expressed in both atrium and ventricle in 48 hpf control embryos and there is decreased expression in (D) morphant embryos. (E) Vmhc delineates ventricle specification at 48 hpf and (F) is decreased in morphant embryos. (G and I) Expression of both notch1b and bmp4 expression was restricted to the atrio-ventricular boundary (black arrows) in control morpholino injected embryo hearts. (H and J) In morphant embryos, both cardiac valve markers were expressed throughout the heart and were not restricted to the atrio-ventricular boundary (white arrows). The numbers of embryos with the displayed phenotype were (A) 62/65 (B) 55/63 (C) 72/75 (D) 53/72 (E) 68/70 (F) 40/72,(G) 77/80, (H) 67/85, (I) 74/78 and (J) 68/74.