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Fig. S1 Circulating definitive proerythroblasts are present in the bloodstream from 4 dpf onwards. (A,B) May-Grunwald Giemsa staining of proerythroblasts isolated from 5 dpf bloodstream (A) and adult kidney marrow (B). (C) The profile of definitive proerythroblasts present in the blood during various developmental stages. The vertical axis indicates the percentage of definitive proerythroblasts in the blood. The horizontal axis indicates the stage of embryos sacrificed for analysis. The blue bars represent wild-type embryo. The green bars represent runx1 morphant. For counting the number of definitive proerythroblasts, circulating blood cells are pooled from 30 embryos of 30 hpf and 20 embryos of the other stages.