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Fig. S1 myog and myod MOs can specifically block translation of their mRNAs. (A,B) Western blot of protein extracts from control (A,B) and myog MO-1 (A) or myod MO (B), showing absence of specific bands at ∼37 kDa from the MO lanes. Myod immunohistochemical stain is also ablated (Hammond et al., 2007) #8381. (C) Immunohistochemistry with Myogenin antibody (brown nuclei) of myog MO-1-injected, myog MO-2-injected and control 15 ss embryos. Dorsal flatmounts of one side of anterior somites, anterior to top. (D) Confocal stacks of tail somites of 24 hpf control and myog MO-1-injected embryos after immunodetection of Myogenin (green nuclei). Lateral view, anterior to left. myog morphants lack the nuclear Myogenin reactivity of control embryos.