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Fig. S3 Maternal Celsr2 is required for epiboly, whereas Lyn-Celsr expression does not result in epiboly defects in wild-type embryo. (A) Wild-type embryos were injected with 300 pg RNA encoding Lyn-Celsr. (B) Embryos from crossing between MZord female with TL wild-type male were injected with 0.4 pmoles each of celsr1a and celsr1b morpholinos. The embryos were fixed at 90% epiboly for β-catenin antibody staining. An arrowhead indicates the leading edge of deep cells. Note that embryos expressing Lyn-Celsr show no epiboly phenotype (n=33).
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