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Fig. 8
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Fig. 8 V2a cells are expanded and V2b cells are lost in mind bomb mutants. Lateral views of spinal cord gene expression in mib mutant trunks at 18 somites (18 h) (A–C) and 24 h (E–L). (D) Wild-type embryo at 24 h. In panel L chx10 expression is shown in red and vsx1 in green. GAD is a marker of GABAergic cells (see Materials and methods). Anterior is left, dorsal is up. Scale bar = 50 μm. scl expression in mib mutants is slightly variable. Some embryos have very few remaining cells, as is shown for gata3 in panel F, but other embryos have slightly more remaining ventral cells as shown in panel G.
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Reprinted from Developmental Biology, 322(2), Batista, M.F., Jacobstein, J., and Lewis, K.E., Zebrafish V2 cells develop into excitatory CiD and Notch signalling dependent inhibitory VeLD interneurons, 263-275, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.