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Fig. 1 Expression patterns of tag1, stbm, and lama1 in the hindbrain during FBMN migration. All panels show dorsal views of the hindbrain with anterior to the left. Asterisks mark the otic vesicle in each panel. (A, C) tag1 is expressed in an increasing number of migrating FBMNs (arrowheads) between 19 hpf (A) and 24 hpf (C). Strongest expression is initially found in cells located in r5/r6 (A), and later in cells spanning r5 and r6 (C). (B) In a 24 hpf Tg(isl1:gfp) embryo, FBMNs (arrowheads) are found throughout the migratory pathway from r4 to r7, with their axons (arrow) exiting the hindbrain in r4. (D) At 24 hpf, Tag1 immunostaining labels only cell bodies of FBMNs located in r6 and r7 (arrowhead), and their axons (arrow). (E) stbm is ubiquitously expressed in the neural tube, and in surrounding non-neural tissues. (F) A 60 μm stack shows broad Laminin1 immunostaining in the basal lamina in the ventral neural tube and adjacent non-neural tissues. tg, trigeminal ganglion; ag, acoustic ganglion; pg, posterior lateral line ganglion. Scale bar in A (75 μm for A, C, E); in B, D, F (75 μm).
Reprinted from Developmental Biology, 325(2), Sittaramane, V., Sawant, A., Wolman, M.A., Maves, L., Halloran, M.C., and Chandrasekhar, A., The cell adhesion molecule Tag1, transmembrane protein Stbm/Vangl2, and Lamininalpha1 exhibit genetic interactions during migration of facial branchiomotor neurons in zebrafish, 363-373, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.