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Fig. 2 (A) dsRed fluorescence overlayed with a brightfield image of a 7 dpf fish reveal the brightly fluorescent cell population in the olfactory bulb (OB) (arrows). (B) The OB cell population in a fish from line sCMV:GFP-2 at 1 month post-fertilization. (C) Horizontal section of the adult transgenic sCMV:dsRed 2-1 fish rostral telencephalon (TEL) and OB stained with the nuclear stain DAPI (blue) and showing fluorescent cell bodies and fibers in the OB (anterior left). (D) Horizontal section of the adult OB revealing dsRed cell bodies predominantly in the glomerular layer (GL) and superficial internal cell layer (ICL) with arbors extending into the olfactory nerve layer (ONL). (E) Consistent with their neuronal morphology, dsRed-positive OB neurons colocalize with HuC/D immunoreactivity (yellow). Insets show individual green (HuC/D) and red (dsRed) channels. (F) Retrograde labeling of olfactory bulb mitral cells (green) reveals dsRed-expressing cells are not mitral cells. (G and H) Higher magnification image shows separate green (G) and red (H) channels. Arrows indicate the position of green-labeled mitral cells in both images. (I) Antibody to tyrosine hydroxylase (green) colocalizes with a subset of dsRed-positive cells (arrows). Insets show individual channels for one double-labeled neuron. All images are of sCMV:dsRed line 2-1. Scale bars: A/B = 250 μm; C = 150 μm; D = 75 μm.
Reprinted from Gene expression patterns : GEP, 9(1), Suhr, S.T., Ramachandran, R., Fuller, C.L., Veldman, M.B., Byrd, C.A., and Goldman, D., Highly-restricted, cell-specific expression of the simian CMV-IE promoter in transgenic zebrafish with age and after heat shock, 54-64, Copyright (2009) with permission from Elsevier. Full text @ Gene Expr. Patterns