|
Fig. 1 (A) Representative pattern of sCMV promoter-driven dsRed expression in a zebrafish embryo 3–4 days following injection of eggs revealing scattered cells throughout the embryo. Top view with brightfield overlay. (B) As in (A), side-view fluorescent image. (C) Top view of the head of control (left and transgenic (right) embryos displaying early dsRed fluorescence in a small population of cells within the rostral telencephalon (arrows indicate cell, boxed region on control shows no positive signal). (D) The same fish as in (C), processed for in situ hybridization and showing reporter mRNA expression in the transgenic fish (right) and a corresponding lack of signal in control siblings (left). Note: oval light-brown patches on both animals are pigment spots and not positive hybridization. (E) Early (2.5 dpf) EGFP expression in rostral telencephalon and other scattered cells in the head, trunk, and spinal cord area of line sCMV:EGFP-1 fish. (F) As in (E), for line sCMV:EGFP-2 that displayed higher expression in spinal cord by 2.5 dpf. (G) Ventral view of the sCMV expression pattern in a 2-week-old sCMV:dsRed 2-1 F1 transgenic fish that represents the general expression pattern observed in young sCMV-transgenic fish. Stable transgenic animals after 7 dpf express most notably in the rostral telencephalon, within the eyes, and in discrete cells of the brain stem and spinal cord.
Reprinted from Gene expression patterns : GEP, 9(1), Suhr, S.T., Ramachandran, R., Fuller, C.L., Veldman, M.B., Byrd, C.A., and Goldman, D., Highly-restricted, cell-specific expression of the simian CMV-IE promoter in transgenic zebrafish with age and after heat shock, 54-64, Copyright (2009) with permission from Elsevier. Full text @ Gene Expr. Patterns