|
Fig. 1 BMP activity is transiently increased during early somitogenesis in Dlx3b/4b morphants. (A,B) bmp4 expression is unaffected in Dlx3b/4b morphants prior to the onset of dlx3b/4b expression in 70% epiboly embryos. (C,D) At bud stage, the domain of bmp4 expression is expanded in the prechordal plate and tailbud of Dlx3b/4b morphants (D). Insets depict the increase of the prechordal domain. (E,F) Antibodies against PSMAD1/5/8 (red) reveal an increase in cells responding to BMP activity of Dlx3b/4b morphants (F). DAPI-stained nuclei are blue. Inset in (F) is a high magnification image depicting PSMAD1/5/8 co-localization with DAPI-stained nuclei. (G,H) bmp4 expression in Dlx3b/4b morphants is comparable to controls at 18-somites. All views are lateral views, with ventral to the left in (A,B), anterior to the left in (C–F), and anterior to the bottom in (G,H). Insets in (C,D) are dorsal views, with anterior to the top.
Reprinted from Developmental Biology, 325(1), Esterberg, R., and Fritz, A., dlx3b/4b are required for the formation of the preplacodal region and otic placode through local modulation of BMP activity, 189-199, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.