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Fig. 5 GnRH3-producing neurons transiently express nelf mRNA. A-H: Staining of Tg(gnrh3:EGFP) embryos with fluorescent enhanced green fluorescent protein (EGFP) antibody (A,D,G) and nelf riboprobe (B,E,H). A single optical slice of confocal micrograph (1.8 μm). Dorsal view, anterior to the top. C,F: The analysis reveals colocalization in the olfactory epithelium at 32 hours postfertilization (hpf; C) and in the terminal nerve at 48 hpf (F). At 72 hpf, GnRH3 neurons no longer express nelf (I). Scale bar = 10 μM.
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